2014 – 2015
Florida Department of Education
Curriculum Framework 

Program Title:	Industrial Biotechnology
Program Type:	Career Preparatory
Career Cluster:	Manufacturing

Secondary – Career Preparatory	
Program Number	8736000	
CIP Number	0626061601	
Grade Level	9-12, 30, 31	
Standard Length	3 credits	
Teacher Certification	BIOLOGY & BIOTECHNICIAN 7G
or
CHEMISTRY & BIOTECHNICIAN 7G	
CTSO	SkillsUSA	
SOC Codes (all applicable) 	19-4021 – Biological Technicians	
Facility Code	245  http://www.fldoe.org/edfacil/sref.asp (State Requirements for Educational Facilities)	
Targeted Occupation List	http://www.labormarketinfo.com/wec/TargetOccupationList.htm	
Perkins Technical Skill Attainment Inventory	http://www.fldoe.org/workforce/perkins/perkins_resources.asp 	
Industry Certifications	http://www.fldoe.org/workforce/fcpea/default.asp 	
Statewide Articulation	http://www.fldoe.org/workforce/dwdframe/artic_frame.asp 	

Purpose 

This program offers a sequence of courses that provides coherent and rigorous content aligned with challenging academic standards and relevant technical knowledge and skills needed to prepare for further education and careers in the manufacturing career cluster; provides technical skill proficiency, and includes competency-based applied learning that contributes to the academic knowledge, higher-order reasoning and problem-solving skills, work attitudes, general employability skills, technical skills, and occupation-specific skills, and knowledge of all aspects of the manufacturing career cluster. 

Industrial Biotechnology is a program offering students science credit through an applied science education, to prepare them for entry level positions in the cutting edge industry of Biotechnology.  While meeting the state standards for science, the coursework will focus on developing science and bio-technical skills that are current and in demand for this rapidly growing occupation.

Upon completion of the integrated program, students will be able to explain and perform bio-technical skills used by Industrial, Medical, Agricultural, and Research facilities that develop and produce marketable products and processes. 

Additional Information relevant to this Career and Technical Education (CTE) program is provided at the end of this document.

Program Structure

This program is a planned sequence of instruction consisting of three occupational completion points.

The following table illustrates the Secondary program structure:

OCP	Course Number	Course Title	Length	SOC Code	Level	
A	3027010	Biotechnology 1	1 credit	19-4021	3	
B	3027020	Biotechnology 2	1 credit	19-4021	3	
C	8736030	Biotechnology 3	1 credit	19-4021	3	

Academic Alignment Table

Some or all of the courses in this program have been aligned to the Next Generation Sunshine State Standards for science and the Florida Standards for mathematics and english/language arts. The table below contains the results of the alignment efforts. Data shown in the table includes: the quantity of academic standards in the CTE course; the total number of standards contained in the academic course; and the percentage of alignment to the CTE course.

Courses	Anatomy/ Physiology
Honors	Astronomy     Solar/Galactic Honors	Biology 1	Chemistry 1	Earth-Space Science	Genetics
Honors	Integrated Science	Marine Science 1 Honors	Physical Science	Physics 1	Environmental Science	
3027010	12/87
14%	21/80
26%	35/83
42%	23/69
33%	10/67
15%	30/69
43%	28/82
34%	20/66
30%	24/74
32%	16/72
22%	19/70
27%	
3027020	11/87
13%	11/80
14%	24/83
29%	14/69
20%	6/67
9%	17/69
25%	18/82
22%	17/66
26%	11/74
15%	8/72
11%	17/70
24%	
8736030	16/87
18%	27/80
34%	35/83
42%	26/69
38%	13/67
19%	29/69
42%	27/82
33%	24/66
36%	27/74
36%	21/72
29%	24/70
34%	
**   Alignment pending review    						#   Alignment attempted, but no correlation to academic course

Courses	Algebra 1	Algebra 2	Geometry	English 1	English 2	English 3	English 4	
3027010	8/67
1%	3/75
4%	2/54
4%	13/49
27%	13/48
27%	#	#	
3027020	10/67
15%	4/75
5%	2/54
4%	11/49
22%	11/48
23%	#	#	
8736030	6/67
9%	4/75
5%	#	#	#	8/45
18%	8/45
18%	
**   Alignment pending review    		            #   Alignment attempted, but no correlation to academic course

Florida Standards for Technical Subjects 

Florida Standards (FS) for English Language Arts and Literacy in History/Social Studies, Science, and Technical Subjects are the critical reading and writing literacy standards designed for grade 6 and above.  These standards are predicated on teachers of history/social studies, science, and technical subjects using their content area expertise to help students meet the particular challenges of reading, writing, speaking, listening, and language in their respective fields.  It is important to note that the 6-12 literacy standards in history/social studies, science, and technical subjects are not meant to replace content standards in those areas but rather to supplement them.

This curriculum framework incorporates the grades 9-10 reading and writing literacy standards in the first two courses of this CTE program and grade 11-12 reading and writing literacy standards in the third and fourth courses of this CTE program.  The standards for Mathematical Practices describe varieties of expertise that educators at all levels should seek to develop in their students.  These practices rest on important “processes and proficiencies” with longstanding importance in mathematics education.  This curriculum framework incorporates the appropriate mathematical practices in the first four courses of this CTE program.  

Florida Standards for Mathematics & Language Arts (FS-M/LA)

Some or all of the courses in this program have been aligned to the Florida Standards for Mathematics and Language Arts used in core academic classes.  Data shown in the framework table (column ‘FS-M/LA’) contains the results of these alignment efforts.

Next Generation Sunshine State Standards (NGSSS) - Science 

Some or all of the courses in this program have been aligned to the Next Generation Sunshine State Standards (NGSSS) for Science.  Data shown in the framework table (column ‘NGSSS-Sci’) contains the results of these alignment efforts.

Common Career Technical Core – Career Ready Practices

Career Ready Practices describe the career-ready skills that educators should seek to develop in their students.  These practices are not exclusive to a Career Pathway, program of study, discipline or level of education.  Career Ready Practices should be taught and reinforced in all career exploration and preparation programs with increasingly higher levels of complexity and expectation as a student advances through a program of study. 

1. Act as a responsible and contributing citizen and employee. 

2. Apply appropriate academic and technical skills. 

3. Attend to personal health and financial well-being. 

4. Communicate clearly, effectively and with reason. 

5. Consider the environmental, social and economic impacts of decisions. 

6. Demonstrate creativity and innovation. 

7. Employ valid and reliable research strategies. 

8. Utilize critical thinking to make sense of problems and persevere in solving them. 

9. Model integrity, ethical leadership and effective management. 

10. Plan education and career path aligned to personal goals. 

11. Use technology to enhance productivity. 

12. Work productively in teams while using cultural/global competence. 

Standards

After successfully completing this program, the student will be able to perform the following:

01.0	Methods and strategies for using Florida Standards for grades 09-10 reading in Technical Subjects for student success in Industrial Biotechnology. 
02.0	Methods and strategies for using Florida Standards for grades 09-10 writing in Technical Subjects for student success in Industrial Biotechnology.
03.0	Methods and strategies for using Florida Standards for grades 09-10 Mathematical Practices in Technical Subjects for student success in Industrial Biotechnology.

Academic Knowledge Standards:
04.0	Apply knowledge of the nature of science and scientific habits of mind to solve problems, and employ safe and effective use of laboratory technologies.
05.0	Demonstrate understanding of the roles of matter, energy, in the chemical processes of cells, organisms.
06.0	Demonstrate an understanding of the structure and processes of the cell, with emphasis on reproduction and communication.
07.0	Demonstrate an understanding of the fundamentals of biochemistry including protein synthesis and reproduction.
08.0	Demonstrate an understanding of genetics, including the principles of, molecular basis, diversity, and applications to biotechnology.
09.0	Demonstrate an understanding of the levels of organization, from atoms to molecular DNA to organisms, classification, taxonomy.
10.0	Demonstrate an understanding of the interdependence of organisms, humans, and the environment.
11.0	Demonstrate an understanding of genetic diversity, selection, adaptations, and changes through time.
12.0	Demonstrate an understanding of the legal and ethical responsibilities associated with working with biological specimens for research or industry, bioethics.
13.0	Demonstrate an understanding of the connection between biotechnology, agricultural, food, and medical technologies and careers.

CTE Performance Standards:
14.0	Demonstrate knowledge of the history, career fields, and benefits of biotechnology. 
15.0	Recognize and practice safety procedures.
16.0	Recognize and follows quality control procedures and regulatory guidelines.
17.0	Demonstrate the ability to communicate and use interpersonal skills effectively.
18.0	Apply basic skills in scientific inquiry, calculations, and analysis.
19.0	Demonstrate knowledge of organism structure and function. 
20.0	Utilize materials processing and standard laboratory operating procedures for biotechnology. 
21.0	Apply biotechnical materials analysis skills. 
22.0	Demonstrates knowledge of basic chemistry as applied to biotechnology procedures. 
23.0	Utilizes basic knowledge of microbiology and blood-borne diseases, including AIDS. 
24.0	Demonstrate knowledge of legal and ethical responsibilities. 
25.0	Demonstrate literacy and computer skills applicable to the biotechnology industry.
26.0	Demonstrate employability skills.

Academic Knowledge Standards:
27.0	Apply knowledge of the nature of science and scientific habits of mind to solve problems, and employ safe and effective use of laboratory technologies.
28.0	Demonstrate understanding of the chemical processes in biotechnology, pH, solutions, dilutions, molarity.
29.0	Demonstrate an understanding of cell propagation, growth and cultures for biotechnology.
30.0	Demonstrate an understanding of the fundamentals of biochemistry including protein synthesis, recombinants, and reproduction, analysis, western blot.
31.0	Demonstrate an understanding of genetics and biotechnology, gene selection, transformation, analysis, PCR, Northern and Southern blot.
32.0	Demonstrate knowledge of the structure and function and reproduction of various organisms used as genetic models.
33.0	Demonstrate an understanding of the interdependence of organisms, humans, and the environment.
34.0	Demonstrate an understanding of genetic diversity, natural and genetic selection.
35.0	Demonstrate an understanding of bioethics.
36.0	Demonstrate and understanding of the connection between Biotechnology, agricultural, food, and medical technologies and careers.

CTE Performance Standards:
37.0	Demonstrate knowledge of the history, career fields, and benefits of biotechnology. 
38.0	Recognize and practice safety procedures. 
39.0	Recognize and follows quality control procedures and regulatory guidelines.
40.0	Demonstrate the ability to communicate and use interpersonal skills effectively. 
41.0	Apply basic skills in scientific inquiry, calculations, and analysis. 
42.0	Demonstrate knowledge of organism structure and function.
43.0	Utilize materials processing and standard laboratory operating procedures for biotechnology. 
44.0	Apply biotechnical materials analysis skills.
45.0	Demonstrates knowledge of basic chemistry as applied to biotechnology procedures. 
46.0	Utilizes basic knowledge of microbiology and blood-borne diseases, including AIDS.
47.0	Demonstrate knowledge of legal and ethical responsibilities.
48.0	Demonstrate literacy and computer skills applicable to the biotechnology industry.
49.0	Demonstrate employability skills. 

CTE Performance Standards:
50.0	Methods and strategies for using Florida Standards for grades 11-12 reading in Technical Subjects for student success in Industrial Biotechnology.
51.0	Methods and strategies for using Florida Standards for grades 11-12 writing in Technical Subjects for student success in Industrial Biotechnology.
52.0	Methods and strategies for using Florida Standards for grades 11-12 Mathematical Practices in Technical Subjects for student success in Industrial Biotechnology.
53.0	History, career fields, and benefits of biotechnology. 
54.0	Safety procedures.
55.0	Quality control procedures and regulatory guidelines.
56.0	Communicate and use interpersonal skills effectively. 
57.0	Basic skills in scientific inquiry, calculations, and analysis. 
58.0	Organism structure and function.
59.0	Materials processing and standard laboratory operating procedures for biotechnology. 
60.0	Biotechnical materials analysis skills. 
61.0	Basic chemistry as applied to biotechnology procedures.
62.0	Microbiology and blood-borne diseases, including AIDS.
63.0	Legal and ethical responsibilities.
64.0	Literacy and computer skills applicable to the biotechnology industry.
65.0	Employability skills.

2014 – 2015
Florida Department of Education
Student Performance Standards

Course Title: 	Biotechnology 1 
Course Number: 	3027010
Course Credit:	1 Science

Course Description:  This course provides exploratory experience combining laboratory and real-life applications in the field of biotechnology.  The content includes, but is not limited to, the following:

•	The nature of science
•	Matter, energy, chemical processes of cells, organisms
•	Cell molecular structure and function, membranes, DNA, plasmids, reproduction, communication
•	Fundamentals of biochemistry, protein synthesis, germ theory, 
•	Molecular genetics and biotechnology, restriction digest, DNA analysis, PCR
•	Levels of organization, molecular to organismal, classification, and taxonomy
•	Interdependence of organisms, humans, and the environment,
•	Genetic diversity, selection, adaptations, and changes through time
•	Bioethics
•	Connection between Biotechnology, agricultural, food, and medicine and careers

This course shall integrate the Goal 3 Student Performance Standards of the Florida System of School Improvement and Accountability as appropriate to the content and processes of the subject matter.

Laboratory investigations that include scientific inquiry, research, measurement, problem solving, emerging technologies, tools and equipment, as well as, experimental quality, and safety procedures will be an integral part of this course. Students will interact with materials and primary sources of data or with secondary sources of data to observe and understand the natural world.  Students will develop an understanding of measurement error, and develop the skills to aggregate, interpret, and present the data and resulting conclusions. Equipment and supplies will be provided to enhance these hands-on experiences for students.  A minimum of 20% of classroom time will be dedicated to laboratory experiences.

Florida Standards	Correlation to CTE Program Standard #	
01.0	Methods and strategies for using Florida Standards for grades 09-10 reading in Technical Subjects for student success in Industrial Biotechnology.		
01.01	Key Ideas and Details		
01.01.1	Cite specific textual evidence to support analysis of science and technical texts, attending to the precise details of explanations or descriptions.                                                                LAFS.910.RST.1.1		
01.01.2	Determine the central ideas or conclusions of a text; trace the text’s explanation or depiction of a complex process, phenomenon, or concept; provide an accurate summary of the text.    
LAFS.910.RST.1.2		
01.01.3	Follow precisely a complex multistep procedure when carrying out experiments, taking measurements, or performing technical tasks, attending to special cases or exceptions defined in the text.
LAFS.910.RST.1.3		
01.02	Craft and Structure		
01.02.1	Determine the meaning of symbols, key terms, and other domain-specific words and phrases as they are used in a specific scientific or technical context relevant to grades 9–10 texts and topics.
LAFS.910.RST.2.4		
01.02.2	Analyze the structure of the relationships among concepts in a text, including relationships among key terms (e.g., force, friction, reaction force, energy).                                                             LAFS.910.RST.2.5		
01.02.3	Analyze the author’s purpose in providing an explanation, describing a procedure, or discussing an experiment in a text, defining the question the author seeks to address.                                      LAFS.910.RST.2.6		
01.03	Integration of Knowledge and Ideas		
01.03.1	Translate quantitative or technical information expressed in words in a text into visual form (e.g., a table or chart) and translate information expressed visually or mathematically (e.g., in an equation) into words.
LAFS.910.RST.3.7		
01.03.2	Assess the extent to which the reasoning and evidence in a text support the author’s claim or a recommendation for solving a scientific or technical problem.
LAFS.910.RST.3.8		
01.03.3	Compare and contrast findings presented in a text to those from other sources (including their own experiments), noting when the findings support or contradict previous explanations or accounts.
LAFS.910.RST.3.9		
01.04	Range of Reading and Level of Text Complexity		
01.04.1	By the end of grade 9, read and comprehend literature [informational texts, history/social studies texts, science/technical texts] in the grades 9–10 text complexity band proficiently, with scaffolding as needed at the high end of the range.
01.04.2	By the end of grade 10, read and comprehend literature [informational texts, history/social studies texts, science/technical texts] at the high end of the grades 9–10 text complexity band independently and proficiently.
LAFS.910.RST.4.10		
02.0	Methods and strategies for using Florida Standards for grades 09-10 writing in Technical Subjects for student success in Industrial Biotechnology.		
02.01	Text Types and Purposes		
02.01.1	Write arguments focused on discipline-specific content.
LAFS.910.WHST.1.1		
02.01.2	Write informative/explanatory texts, including the narration of historical events, scientific procedures/experiments, or technical processes.
LAFS.910.WHST.1.2		
02.02	Production and Distribution of Writing		
02.02.1	Produce clear and coherent writing in which the development, organization, and style are appropriate to task, purpose, and audience.
LAFS.910.WHST.2.4		
02.02.2	Develop and strengthen writing as needed by planning, revising, editing, rewriting, or trying a new approach, focusing on addressing what is most significant for a specific purpose and audience.
LAFS.910.WHST.2.5		
02.02.3	Use technology, including the Internet, to produce, publish, and update individual or shared writing products, taking advantage of technology’s capacity to link to other information and to display information flexibly and dynamically.
LAFS.910.WHST.2.6		
02.03	Research to Build and Present Knowledge		
02.03.1	Conduct short as well as more sustained research projects to answer a question (including a self-generated question) or solve a problem; narrow or broaden the inquiry when appropriate; synthesize multiple sources on the subject, demonstrating understanding of the subject under investigation.						 LAFS.910.WHST.3.7		
02.03.2	Gather relevant information from multiple authoritative print and digital sources, using advanced searches effectively; assess the usefulness of each source in answering the research question; integrate information into the text selectively to maintain the flow of ideas, avoiding plagiarism and following a standard format for citation.            
LAFS.910.WHST.3.8		
02.03.3	Draw evidence from informational texts to support analysis, reflection, and research.
LAFS.910.WHST.3.9		
02.04	Range of Writing		
02.04.1	Write routinely over extended time frames (time for reflection and revision) and shorter time frames (a single sitting or a day or two) for a range of discipline-specific tasks, purposes, and audiences.
LAFS.910.WHST.4.10		
03.0	Methods and strategies for using Florida Standards for grades 09-10 Mathematical Practices in Technical Subjects for student success in Industrial Biotechnology.		
03.01	Make sense of problems and persevere in solving them.
MAFS.K12.MP.1.1		
03.02	Reason abstractly and quantitatively.
MAFS.K12.MP.2.1		
03.03	Construct viable arguments and critique the reasoning of others.
MAFS.K12.MP.3.1		
03.04	Model with mathematics.
MAFS.K12.MP.4.1		
03.05	Use appropriate tools strategically.
MAFS.K12.MP.5.1		
03.06	Attend to precision.
MAFS.K12.MP.6.1		
03.07	Look for and make use of structure.
MAFS.K12.MP.7.1		
03.08	Look for and express regularity in repeated reasoning.
MAFS.K12.MP.8.1		

Abbreviations: 
FS-M/LA = Florida Standards for Math/Language Arts
NGSSS-Sci = Next Generation Sunshine State Standards for Science

Note:  This course is pending alignment in the following categories:  National Standards

CTE Standards and Benchmarks	FS-M/LA	NGSSS-Sci	National Standards	
Academic Knowledge Standards:				
These standards support the following Florida Standards and Next Generation Sunshine State Standards:	MAFS.912.S-IC.2	SC.912.L.14.1, 2,  3; 16.3,  5,  8,  9,  10,  11, 14,  16,  17;  17.14;  18.1, 11
SC.912.N.1.1,  2,  3,  4, 5,  6,  7;  2.1,  2,  5;  3.4, 5;  4.1,  2
SC.912.P.8.6,  7		
04.0	Apply knowledge of the nature of science and scientific habits of mind to solve problems, and employ safe and effective use of laboratory technologies--The student will be able to:				
04.01	Know that investigations are conducted to explore new phenomena, to check on previous results, to test how well a theory predicts, and to compare different theories. 				
04.02	Know that from time to time, major shifts occur in the scientific view of how the world works, but that more often, the changes that take place in the body of scientific knowledge are small modifications of prior knowledge.				
04.03	Understand that no matter how well one theory fits observations, a new theory might fit them as well or better, or might fit a wider range of observations, because in science, the testing, revising, and occasional discarding of theories, new and old, never ends and leads to an increasingly better understanding of how things work in the world, but not to absolute truth. 	LAFS.910.SL.1.1			
04.04	Know that the potential for bias exists within individuals and scientific teams, and therefore scientists are expected to seek out possible sources of bias in the design of their investigations and in their data analysis. 	LAFS.910.RI.3.8			
04.05	Understand that new ideas in science are limited by the context in which they are conceived, are often rejected by the scientific establishment, sometimes spring from unexpected findings, and usually grow slowly from many contributors. 	LAFS.910.SL.1.1			
04.06	Understand that in the short run, new ideas that do not mesh well with mainstream ideas in science often encounter vigorous criticism and that in the long run, theories are judged by how they fit with other theories, the range of observations they explain, how well they explain observations, and how effective they are in predicting new findings.	LAFS.910.SL.1.1			
04.07	Know that scientists assume that the universe is a vast system in which basic rules exist that may range from very simple to extremely complex but that scientists operate on the belief that the rules can be discovered by careful, systemic study. 				
04.08	Understand the difference between laws and theories.	LAFS.910.L.3.6			
05.0	Demonstrate understanding of the roles of matter, energy, in the chemical processes of cells, organisms--The student will be able to:				
05.01	Knows that the electron configuration in atoms determines how a substance reacts and how much energy is involved in its reactions. 				
05.02	Knows that the vast diversity of the properties of materials is primarily due to variations in the forces that hold molecules together.				
05.03	Knows that a change from one phase of matter to another involves a gain or loss of energy. 				
05.04	Knows that connections (bonds) form between substances when outer-shell electrons are either transferred or shared between their atoms, changing the properties of substances. 				
05.05	Knows that the number and configuration of electrons will equal the number of protons in an electrically neutral atom and when an atom gains or loses electrons the charge is unbalanced.				
05.06	Knows the difference between an element, a molecule, ion, and a compound 	LAFS.910.L.3.6			
05.07	Knows that elements are arranged into groups and families based on similarities in electron structure and that their physical and chemical properties can be predicted. 				
05.08	Understand how knowledge of energy is fundamental to all the scientific disciplines (e.g., the energy required for biological processes in living organisms and the energy required for the building, erosion, and rebuilding of the Earth). 	LAFS.910.W.1.1			
05.09	Understand that there is conservation of mass and energy when matter is transformed. 				
05.10	Understand that biological systems obey the same laws of conservation as physical systems. 				
05.11	Knows that organisms respond to internal and external stimuli. 				
06.0	Demonstrate an understanding of the structure and processes of the cell, with emphasis on reproduction and communication--The student will be able to:				
06.01	Knows that body structures are uniquely designed and adapted for their function. 				
06.02	Knows that cell behavior can be affected by molecules from other parts of the organism or even from other organisms, and the environment.				
07.0	Demonstrate an understanding of the fundamentals of biochemistry including protein synthesis and reproduction--The student will be able to:				
07.01	Know that body processes involve specific biochemical reactions governed by biochemical principles, and that pathways have been identified through advances in molecular analyses, which have led to better understanding, diagnosis, and treatment of disease. 				
07.02	Know that membranes are sites for chemical synthesis and essential energy conversions. 				
07.03	Know the complex interactions among the different kinds of molecules in the cell cause distinct cycles of activity governed by proteins. 				
08.0	Demonstrate an understanding of genetics, including the principles of, molecular basis, diversity, and applications to biotechnology--The student will be able to:				
08.01	Understands the mechanism of asexual and sexual reproduction and knows the different genetic advantages and disadvantages of sexual and asexual reproduction. 				
08.02	Knows that every cell contains a “blueprint” coded in DNA molecules that specify how proteins are assembled to regulate cells.				
09.0	Demonstrate an understanding of the levels of organization, from atoms to molecular DNA to organisms, classification, taxonomy--The student will be able to:				
09.01	Knows that chemical elements that make up the molecules of living things are combined and recombined in different ways. 				
10.0	Demonstrate an understanding of the interdependence of organisms, humans, and the environment--The student will be able to:				
10.01	Understands the interconnectedness of the systems on Earth and the quality of life. 	LAFS.910.SL.1.1			
10.02	Knows of the great diversity and interdependence of living things. 				
10.03	Know that changes in a component of an ecosystem will have unpredictable and predictable effects on the entire system, but that the components of the system tend to react in a way that will restore the ecosystem to its original condition.				
11.0	Demonstrate an understanding of genetic diversity, selection, adaptations, and changes through time--The student will be able to:				
11.01	Understand the mechanisms of change (e.g., mutation and natural selection) that lead to adaptations in a species and their ability to survive naturally in changing conditions and to increase species diversity. 	MAFS.912.S-CP.1.2			
11.02	Know of the great diversity and interdependence of living things. 				
11.03	Understands how genetic variation of offspring contributes to natural selection. 	MAFS.912.S-ID.1.1;  2.5 			
12.0	Demonstrate an understanding of the legal and ethical responsibilities associated with working with biological specimens for research or industry, bioethics--The student will be able to:				
12.01	Understand the importance of a sense of responsibility, a commitment to peer review, truthful reporting of the methods and outcomes of investigations, and making the public aware of the findings. 				
12.02	Know that scientists first define then control conditions in order to obtain evidence, but when that is not possible for practical or ethical reasons, they try to observe a wide range of natural occurrences to discern patterns. 				
12.03	Know that performance testing is often conducted using small-scale models, computer simulations, or analogous systems to reduce the chance of system failure. 				
12.04	Know that scientific knowledge is used by those who engage in design and technology to solve practical problems, taking human values and limitations into account. 				
12.05	Discuss the extended impact of involving animal and human subjects in research with respect to humane treatment, providing full disclosure to clinical trial participants, ensuring patient confidentiality, and obtaining familial consent.	LAFS.910.SL.1.1			
13.0	Demonstrate an understanding of the connection between biotechnology, agricultural, food, and medical technologies and careers--The student will be able to:				
13.01	Know that scientists can bring information, insights, and analytical skills to matters of public concern and help people understand the possible causes and effects of events. 				
13.02	Know that funds for science research come from federal government agencies, industry, and private foundations and that this funding often influences the areas of discovery. 				
				
CTE Performance Standards:				
14.0	Demonstrate knowledge of the history, career fields, and benefits of biotechnology--The student will be able to:		SC.912.N.4.2		
14.01	Describe major historic developments in biotechnology fields such as pharmaceuticals, biopharmaceuticals, agriculture, diagnostics, industrial products, devices, instrumentation, and research and development. 	LAFS.910.SL.2.4			
14.02	Identify several products obtained through recombinant DNA technology and other biotechnology advances. 				
14.03	Describe the major steps in a product’s move through a company’s product pipeline. 	LAFS.910.RI.1.2			
14.04	Explain how companies decide on the research and development targets and potential products. 				
14.05	Give examples of how the biotechnology revolution has had an impact on current science and manufacturing practices, as well as how new discoveries in science have in turn impacted biotechnology. 	LAFS.910.SL.2.4			
14.06	Illustrate examples of how biotechnology has led to benefits and risks to society and how biotechnical advances affect human lives on a personal level. 	LAFS.910.W.1.2 LAFS.910.RI.1.1			
15.0	Recognize and practice safety procedures--The student will be able to:				
15.01	Identify safety symbols and signs. 				
15.02	Identify appropriate safety procedures and guidelines.				
15.03	Demonstrate an understanding of the emergency procedures in case of fire, burn, chemical spill or other hazardous situations. 	LAFS.910.W.1.2			
15.04	Recognize laboratory safety hazards and avoid them. 				
15.05	Locate and identify emergency equipment, including first aid. 				
15.06	Use laboratory apparatus, materials, and technology in an appropriate and safe manner.				
15.07	Locate a Material Safety Data Sheet (MSDS) and use the information to operate in a safe manner. 				
15.08	Demonstrate knowledge of universal precautions for blood-borne pathogens. 	LAFS.910.W.1.2			
16.0	Recognize and follows quality control procedures and regulatory guidelines--The student will be able to:				
16.01	Identify the need for and function of regulatory agencies such as those in government, industry, and society. 	LAFS.910.RI.1.1			
16.02	Describe appropriate attire for different biotechnology workplaces including the office, laboratory and cleanroom environments.				
16.03	Monitor, use, store and dispose of hazardous materials properly. 				
16.04	Clean, organize, and sterilize materials and equipment. 				
17.0	Demonstrate the ability to communicate and use interpersonal skills effectively--The student will be able to:				
17.01	Follow all oral and written instructions. 	LAFS.910.SL.1.1
LAFS.910.W.1.2			
17.02	Demonstrate good listening, writing, and verbal communication skills and procedures.				
17.03	Appropriately use and respond to verbal and non-verbal cues. 	LAFS.910.W.2.4			
17.04	Use correct spelling, grammar, and format in all written communication. 	LAFS.910.L.3.6			
17.05	Use appropriate scientific terminology and abbreviations. 				
17.06	Recognize the importance of courtesy and respect and maintain good interpersonal relationships.	LAFS.910.RI.1.1
LAFS.910.SL1.1			
17.07	Read and discuss technical material.	LAFS.910.W.1.2			
17.08	Read and present a scientific paper for discussion, including an overview of the objective, experimental methods, results, and conclusions.	LAFS.910.SL.1.1 LAFS.910.W.1.2			
18.0	Apply basic skills in scientific inquiry, calculations, and analysis--The student will be able to:	MAFS.912.S-IC.2	SC.912.N.1.1,  2		
18.01	Demonstrate knowledge of the scientific method. 				
18.02	Use a variety of methods including literature searches in libraries, computer databases, or on-line, for gathering background information, making observations, and collecting and organizing data.	LAFS.910.W.3.7			
18.03	Use the scientific method to conduct a valid experiment, including hypothesis formation, data collection, data analysis including results and discussion, and conclusion. 	
LAFS.910.W.2.4 LAFS.910.SL.1.1
MAFS.912.S-ID.1.1,  2,  3			
18.04	Maintain a scientific notebook that includes all laboratory procedures, data, and conclusions. 				
18.05	Properly and safely operate scientific equipment including graduates, hoods, microscopes, pipets, micropipets, electronic balance, pH meters, incubators, centrifuges, water baths, power supplies and electrophoresis chambers.  	LAFS.910.W.2.4			
18.06	Make and use measurements in both traditional and metric units. 	MAFS.912.N-Q.1.3			
18.07	Measure time, temperature, distance, capacity and mass/weight. 	MAFS.912.N-Q.1.3			
18.08	Describe the relationship between 12 and 24 hour time and be able to convert between the two. 				
18.09	Make estimates and approximations in order to test the reasonableness of the result. 	MAFS.912.N-Q.1.3			
18.10	Evaluate the validity of results obtained during experimentation and product development.  	MAFS.912.S-ID.1.1,  2,  3			
18.11	Interpret and use graphs, charts and tables used to collect and analyze data. 	MAFS.912.S-ID.1.1,  2,  3			
18.12	Interpret quantitative and qualitative data. 	LAFS.910.W.1.2
MAFS.912.S-ID.1.1,  2,  3			
18.13	Demonstrate ability to evaluate and draw conclusions. 	LAFS.910.W.1.2			
18.14	Follow guidelines to prepare a scientific report.	LAFS.910.W.1.2			
19.0	Demonstrate knowledge of organism structure and function--The student will be able to:		SC.912.L.14.1,  2 , 3;  15.6, 15;  16.1,  2,  3,  4, 5,  6,  7,  9,  14,  16, 17;  18.1,  7,  8,  9		
19.01	Recognize and distinguish between the following based upon the hierarchy of organization of organisms: atom, molecule, cells, tissue, organs, organ system, and organism. 	LAFS.910.L.3.6			
19.02	Outline the life cycle and characteristics of certain model organisms used in the biotechnology industry, including bacterial, yeast, and mammalian cells, and viruses. 	LAFS.910.RI.1.3			
19.03	Differentiate between prokaryotic and eukaryotic cells. 	LAFS.910.RI.1.2			
19.04	Describe the cell (both prokaryotes and eukaryotes) as the basic unit of life. 	LAFS.910.RI.1.2			
19.05	Analyze the difference between plant and animal cells. 	LAFS.910.SL.1.1 LAFS.910.RI.1.2			
19.06	Describe cell structure and function. 	LAFS.910.L.3.6 LAFS.910.SL.1.1			
19.07	Differentiate between mitosis and meiosis. 	LAFS.910.L.3.6			
19.08	Describe the role of DNA, RNA, and ribosomes in protein synthesis. 	LAFS.910.W.1.2			
20.0	Utilize materials processing and standard laboratory operating procedures for biotechnology--The student will be able to: 		SC.912.L.14.6 SC.912.N.1.1		
20.01	Maintain a clean and organized work area.				
20.02	Follow written protocols and oral directions to perform a variety of laboratory and technical tasks. 				
20.03	Determine appropriate equipment and units of measurement for a given task. 	MAFS.912.N-Q.1.3			
20.04	Discuss and perform disinfection and sterilization techniques. 	LAFS.910.SL.1.1			
21.0	Apply biotechnical materials analysis skills--The student will be able to:		SC.912.L.14.3 SC.912.L.16.11,  12 SC.912.L.17.13,  20		
21.01	Isolate DNA from a variety of cells. 				
21.02	Explain the principles involved in agarose gel electrophoresis. 	LAFS.910.W.1.2			
21.03	Prepare, load, run, visualize, and analyze DNA samples on an agarose gel. 				
21.04	Describe the meaning in differences in DNA and peptide bands seen on agarose gels.  	LAFS.910.W.1.2			
21.05	Explain the difference between analyzing PCR products on conventional gels vs. using a Realtime PCR system.				
21.06	Discuss sources of environmental contamination and methods of detection in controlled environments. 	LAFS.910.SL.1.1			
22.0	Demonstrates knowledge of basic chemistry as applied to biotechnology procedures--The student will be able to:		SC.912.N.3.5 SC.912.P.8.4,  5,  6,  7,  8,  9; 12.12		
22.01	Recognize and provide labels for models of neutral atoms, ions, and isotopes. 				
22.02	Differentiate between atoms, elements, molecules, compounds, mixtures and solutions. 	LAFS.910.L.3.6			
22.03	Compare and contrast homogenous and heterogeneous solutions and suspensions. 	LAFS.910.L.3.6			
22.04	Determine chemical characteristics and reactivity based on electron configuration. 				
22.05	Demonstrate that the rate of chemical reactions depend on reactant concentration or temperature, or the presence of a catalyst. 	MAFS.912.S-ID.1.1,  2; 3.7			
22.06	Explain how the electron configuration determines covalent and ionic bonds. 				
22.07	Compare and contrast the variety of forces that hold matter together. 	LAFS.910.L.3.6			
23.0	Utilizes basic knowledge of microbiology and blood-borne diseases, including AIDS--The student will be able to:		SC.912.L.14.3,  6 SC.912.L.15.5,  6		
23.01	Differentiate between aerobic vs. anaerobic bacteria, viruses, bacteriophage, and mycoplasma.	LAFS.910.RI.1.1			
23.02	Discuss microbial taxonomy and classification. 	LAFS.910.RI.1.1			
23.03	Practice aseptic techniques as required. 				
23.04	Discuss sterilization and isolation techniques. 				
23.05	Discuss techniques of inoculation and transfer of cultures. 				
23.06	Describe conditions that promote cell growth under aseptic conditions in the laboratory and workplace. 				
23.07	Identify "at risk" behaviors which promote the spread of diseases caused by blood borne pathogens. 	LAFS.910.W.3.7			
23.08	Discuss differences between sterilization, decontamination, and disinfection.	LAFS.910.RI.1.1			
23.09	Demonstrate proper protocol for the disposal of biohazardous waste and microorganisms.				
24.0	Demonstrate knowledge of legal and ethical responsibilities--The student will be able to:		SC.912.L.16.10		
24.01	Discuss ethical, legal and social issues raised by biotechnology. 	LAFS.910.W.3.7			
25.0	Demonstrate literacy and computer skills applicable to the biotechnology industry--The student will be able to:				
25.01	Define terms and demonstrate basic computer skills. 				
25.02	Describe the uses of computers in the biotechnology industry. 				
25.03	Use the Internet to gather and share scientific and regulatory information. 	LAFS.910.W.2.6			
25.04	Use spreadsheet software to calculate and analyze data. 	MAFS.912.S-ID.1.1,  2,  3,  4			
26.0	Demonstrate employability skills--The student will be able to:				
26.01	Demonstrate appropriate responses to criticism and coaching from employer, supervisor, or other persons.	LAFS.910.W.2.5			
26.02	Demonstrate appropriate methods for asking questions, and providing constructive criticism and feedback.				
26.03	Use several resources including the internet to gather information about job opportunities in the biotechnology field. 	LAFS.910.W.3.7			
26.04	Outline the opportunities for careers in biotechnology in health, industry, medicine, genetics, agriculture, etc. 	LAFS.910.RI.1.2			
26.05	Identify and demonstrate acceptable work habits and health habits. 				
26.06	Follow acceptable work habits, personal characteristics and hygiene habits for the biotechnology workplace.				

2014 – 2015
Florida Department of Education
Student Performance Standards

Course Title: 	Biotechnology 2 
Course Number: 	3027020
Course Credit:	1 Science

Course Description: This course provides exploratory experience combining laboratory and real-life applications in the field of biotechnology.  The content includes, but is not limited to, the following:

•	The nature of science
•	Chemical processes in biotechnology, pH, solutions, molarity
•	Cell propagation, growth and cultures for biotechnology
•	Biochemistry, proteins, enzymes, plasmids, recombinants, blood borne pathogens
•	Genetics and biotechnology, gene selection, transformation, analysis
•	Structure and function of various organisms used as genetic models
•	Interdependence of organisms, humans, and the environment,
•	Genetic diversity and selection
•	Connection between biotechnology, agricultural, food, and medicine  and careers
•	Bioethics

This course shall integrate the Goal 3 Student Performance Standards of the Florida System of School Improvement and Accountability as appropriate to the content and processes of the subject matter.

Prerequisite of this course is Biotechnology in addition to the co-requisite or prerequisite of Biology 1, Honors Biology, or Biology Technology.

Laboratory investigations that include scientific inquiry, research, measurement, problem solving, emerging technologies, tools and equipment, as well as, experimental quality, and safety procedures will be an integral part of this course. Students will interact with materials and primary sources of data or with secondary sources of data to observe and understand the natural world.  Students will develop an understanding of measurement error, and develop the skills to aggregate, interpret, and present the data and resulting conclusions. Equipment and supplies will be provided to enhance these hands-on experiences for students.  A minimum of 20% of classroom time will be dedicated to laboratory experiences.

Florida Standards	Correlation to CTE Program Standard #	
01.0	Methods and strategies for using Florida Standards for grades 09-10 reading in Technical Subjects for student success in Industrial Biotechnology.		
01.01	Key Ideas and Details		
01.01.1	Cite specific textual evidence to support analysis of science and technical texts, attending to the precise details of explanations or descriptions.
LAFS.910.RST.1.1		
01.01.2	Determine the central ideas or conclusions of a text; trace the text’s explanation or depiction of a complex process, phenomenon, or concept; provide an accurate summary of the text.
LAFS.910.RST.1.2		
01.01.3	Follow precisely a complex multistep procedure when carrying out experiments, taking measurements, or performing technical tasks, attending to special cases or exceptions defined in the text.
LAFS.910.RST.1.3		
01.02	Craft and Structure		
01.02.1	Determine the meaning of symbols, key terms, and other domain-specific words and phrases as they are used in a specific scientific or technical context relevant to grades 9–10 texts and topics.
LAFS.910.RST.2.4		
01.02.2	Analyze the structure of the relationships among concepts in a text, including relationships among key terms (e.g., force, friction, reaction force, energy).
LAFS.910.RST.2.5		
01.02.3	Analyze the author’s purpose in providing an explanation, describing a procedure, or discussing an experiment in a text, defining the question the author seeks to address.                                      
LAFS.910.RST.2.6		
01.03	Integration of Knowledge and Ideas		
01.03.1	Translate quantitative or technical information expressed in words in a text into visual form (e.g., a table or chart) and translate information expressed visually or mathematically (e.g., in an equation) into words.
LAFS.910.RST.3.7		
01.03.2	Assess the extent to which the reasoning and evidence in a text support the author’s claim or a recommendation for solving a scientific or technical problem.
LAFS.910.RST.3.8		
01.03.3	Compare and contrast findings presented in a text to those from other sources (including their own experiments), noting when the findings support or contradict previous explanations or accounts.
LAFS.910.RST.3.9		
01.04	Range of Reading and Level of Text Complexity		
01.04.1	By the end of grade 9, read and comprehend literature [informational texts, history/social studies texts, science/technical texts] in the grades 9–10 text complexity band proficiently, with scaffolding as needed at the high end of the range.
01.04.2	By the end of grade 10, read and comprehend literature [informational texts, history/social studies texts, science/technical texts] at the high end of the grades 9–10 text complexity band independently and proficiently.
LAFS.910.RST.4.10		
02.0	Methods and strategies for using Florida Standards for grades 09-10 writing in Technical Subjects for student success in Industrial Biotechnology.		
02.01	Text Types and Purposes		
02.01.1	Write arguments focused on discipline-specific content.
LAFS.910.WHST.1.1		
02.01.2	Write informative/explanatory texts, including the narration of historical events, scientific procedures/experiments, or technical processes.
LAFS.910.WHST.1.2		
02.02	Production and Distribution of Writing		
02.02.1	Produce clear and coherent writing in which the development, organization, and style are appropriate to task, purpose, and audience.
LAFS.910.WHST.2.4		
02.02.2	Develop and strengthen writing as needed by planning, revising, editing, rewriting, or trying a new approach, focusing on addressing what is most significant for a specific purpose and audience.
LAFS.910.WHST.2.5		
02.02.3	Use technology, including the Internet, to produce, publish, and update individual or shared writing products, taking advantage of technology’s capacity to link to other information and to display information flexibly and dynamically.						 LAFS.910.WHST.2.6		
02.03	Research to Build and Present Knowledge		
02.03.1	Conduct short as well as more sustained research projects to answer a question (including a self-generated question) or solve a problem; narrow or broaden the inquiry when appropriate; synthesize multiple sources on the subject, demonstrating understanding of the subject under investigation.                                                           
LAFS.910.WHST.3.7		
02.03.2	Gather relevant information from multiple authoritative print and digital sources, using advanced searches effectively; assess the usefulness of each source in answering the research question; integrate information into the text selectively to maintain the flow of ideas, avoiding plagiarism and following a standard format for citation.
LAFS.910.WHST.3.8		
02.03.3	Draw evidence from informational texts to support analysis, reflection, and research.
LAFS.910.WHST.3.9		
02.04	Range of Writing		
02.04.1	Write routinely over extended time frames (time for reflection and revision) and shorter time frames (a single sitting or a day or two) for a range of discipline-specific tasks, purposes, and audiences.
LAFS.910.WHST.4.10		
03.0	Methods and strategies for using Florida Standards for grades 09-10 Mathematical Practices in Technical Subjects for student success in Industrial Biotechnology.		
03.01	Make sense of problems and persevere in solving them.
MAFS.K12.MP.1.1		
03.02	Reason abstractly and quantitatively.
MAFS.K12.MP.2.1		
03.03	Construct viable arguments and critique the reasoning of others.
MAFS.K12.MP.3.1		
03.04	Model with mathematics.
MAFS.K12.MP.4.1		
03.05	Use appropriate tools strategically.
MAFS.K12.MP.5.1		
03.06	Attend to precision.
MAFS.K12.MP.6.1		
03.07	Look for and make use of structure.
MAFS.K12.MP.7.1		
03.08	Look for and express regularity in repeated reasoning.
MAFS.K12.MP.8.1		

Abbreviations: 
FS-M/LA = Florida Standards for Math/Language Arts
NGSSS-Sci = Next Generation Sunshine State Standards for Science

Note:  This course is pending alignment in the following categories:  National Standards

CTE Standards and Benchmarks	FS-M/LA	NGSSS-Sci	National Standards	
Academic Knowledge Standards:				
27.0	Apply knowledge of the nature of science and scientific habits of mind to solve problems, and employ safe and effective use of laboratory technologies--The student will be able to:				
27.01	Know that investigations are conducted to explore new phenomena, to check on previous results, to test how well a theory predicts, and to compare different theories. 				
27.02	Know that from time to time, major shifts occur in the scientific view of how the world works, but that more often, the changes that take place in the body of scientific knowledge are small modifications of prior knowledge. 				
27.03	Understand that no matter how well one theory fits observations, a new theory might fit them as well or better, or might fit a wider range of observations, because in science, the testing, revising, and occasional discarding of theories, new and old, never ends and leads to an increasingly better understanding of how things work in the world, but not to absolute truth.	LAFS.910.SL.1.1			
27.04	Know that the potential for bias exists within individuals and scientific teams, and therefore scientists are expected to seek out possible sources of bias in the design of their investigations and in their data analysis. 	LAFS.910.RI.3.8			
27.05	Understand that new ideas in science are limited by the context in which they are conceived, are often rejected by the scientific establishment, sometimes spring from unexpected findings, and usually grow slowly from many contributors. 	LAFS.910.SL.1.1			
27.06	Understand that in the short run, new ideas that do not mesh well with mainstream ideas in science often encounter vigorous criticism and that in the long run, theories are judged by how they fit with other theories, the range of observations they explain, how well they explain observations, and how effective they are in predicting new findings. 
	LAFS.910.SL.1.1			
27.07	Know that scientists assume that the universe is a vast system in which basic rules exist that may range from very simple to extremely complex but that scientists operate on the belief that the rules can be discovered by careful, systemic study. 				
28.0	Demonstrate understanding of the chemical processes in biotechnology, pH, solutions, dilutions, molarity--The student will be able to:				
28.01	Experiments and determines that the rates of reaction among atoms and molecules depend on the concentration, pressure, and temperature of the reactants and the presence of absence of catalysts. 	MAFS.912.S-ID1.1,  2; 3.7 			
28.02	Understand how knowledge of energy is fundamental to all the scientific disciplines (e.g., the energy required for biological processes in living organisms and the energy required for the building, erosion, and rebuilding of the Earth). 	LAFS.910.W.1.1			
28.03	Understand that there is conservation of mass and energy when matter is transformed. 				
28.04	Knows that membranes are sites for chemical synthesis and essential energy conversions.				
28.05	Understands that biological systems obey the same laws of conservation as physical systems. 				
29.0	Demonstrate an understanding of cell propagation, growth and cultures for biotechnology--The student will be able to:				
29.01	Understand the mechanisms of asexual and sexual reproduction and know the different genetic advantages and disadvantages of asexual and sexual reproduction. 				
30.0	Demonstrate an understanding of the fundamentals of biochemistry including protein synthesis, recombinants, and reproduction, analysis, western blot--The student will be able to:				
30.01	Define monoclonal antibodies and hybridoma technology.	LAFS.910.L.3.6			
30.02	Know the complex interactions among the different kinds of molecules in the cell cause distinct cycles of activity governed by proteins. 	LAFS.910.RI.1.1			
30.03	Know that cell behavior can be affected by molecules from other parts of the organism or even from other organisms.	LAFS.910.RI.1.1			
31.0	Demonstrate an understanding of genetics and biotechnology, gene selection, transformation, analysis, PCR, northern and southern blot--The student will be able to:				
31.01	Know that the chemical elements that make up the molecules of living things are combined and recombined in different ways. 	LAFS.910.RI.1.1			
31.02	Knows that every cell contains a “blueprint” coded in DNA molecules that specify how proteins are assembled to regulate cells. 	LAFS.910.RI.1.1			
32.0	Demonstrate a knowledge of the structure and function and reproduction of various organisms used as genetic models--The student will be able to:				
32.01	Know that body structures are uniquely designed and adapted for their function. 	LAFS.910.RI.1.1			
32.02	Describe animal models used in research, and the types of studies they are optimally used for.	LAFS.910.RI.1.2			
33.0	Demonstrate an understanding of the interdependence of organisms, humans, and the environment--The student will be able to:				
33.01	Understands the interconnectedness of the systems on Earth and the quality of life. 	LAFS.910.SL.1.1			
33.02	Know that changes in a component of an ecosystem will have unpredictable effects on the entire system, but that the components of the system tend to react in a way that will restore the ecosystem to its original condition. 				
34.0	Demonstrate an understanding of genetic diversity, natural and genetic selection--The student will be able to: 				
34.01	Understand the mechanisms of change (e.g., mutation and natural selection) that lead to adaptations in a species and their ability to survive naturally in changing conditions and to increase species diversity. 	MAFS.912.S-CP.1.2			
34.02	Know of the great diversity and interdependence of living things, and the value of biodiversity. 				
34.03	Understands how genetic variation of offspring contributes to natural selection. 	MAFS.912.S-ID.1.1;  2.5			
35.0	Demonstrate an understanding of bioethics--The student will be able to:				
35.01	Understand the importance of a sense of responsibility, a commitment to peer review, truthful reporting of the methods and outcomes of investigations, and making the public aware of the findings.				
35.02	Know that scientists first define then control conditions in order to obtain evidence, but when that is not possible for practical or ethical reasons, they try to observe a wide range of natural occurrences to discern patterns.				
35.03	Know that performance testing is often conducted using small-scale models, computer simulations, or analogous systems to reduce the chance of system failure. 				
35.04	Know that scientific knowledge is used by those who engage in design and technology to solve practical problems, taking human values and limitations into account. 				
36.0	Demonstrate and understanding of the connection between Biotechnology, agricultural, food, and medical technologies and careers--The student will be able to:				
36.01	Know that funds for science research come from federal government agencies, industry, and private foundations and that this funding often influences the areas of discovery. 	LAFS.910.RI.1.1			
36.02	Know that scientists can bring information, insights, and analytical skills to matters of public concern and help people understand the possible causes and effects of events. 				
36.03	Discuss how scientists contribute to and promote science-based policy in US government.	LAFS.910.SL.1.1			
36.04	Discuss the correlation between scientific discovery and product development, based on societal benefit vs. financial benefit to a company.	LAFS.910.SL.1.1			
				
CTE Performance Standards:				
37.0	Demonstrate knowledge of the history, career fields, and benefits of biotechnology--The student will be able to:		SC.912.E.6.6 SC.912.L.16.10;  17.11 SC.912.N.2.5		
37.01	Understand that technologies used in biotechnology continue to be developed and improved, with respect to advances in discoveries, applications, and demand for increased efficiency.	LAFS.910.RI.1.1			
37.02	Compare the developments in two biotechnology fields and make predictions for future developments in those areas.	LAFS.910.W.3.7			
37.03	Identify several local biotechnology companies specializing in the production of pharmaceuticals, agricultural products, industrial products, and research instruments and reagents. 	LAFS.910.W.3.7			
37.04	Compare the benefits of products derived from biotechnological advances (including DNA technology), to an environment devoid of biotechnological products or applications.	LAFS.910.RI.1.2			
37.05	Demonstrate knowledge of various career fields in the biotechnology industry, including but not limited to research and development, pre-clinical and clinical trials, manufacturing, facilities and equipment, quality control, quality assurance, regulatory affairs, marketing, and sales.	LAFS.910.W.3.7			
38.0	Recognize and practice safety procedures--The student will be able to:		SC.912.L.14.6 SC.912.N.1.1		
38.01	Define Biological Safety Levels 1 through 4 and their differences.	LAFS.910.RI.1.1			
38.02	Describe appropriate attire for Biological Safety Levels 1 through 4.	LAFS.910.RI.1.1			
38.03	Identify potential biohazards and relate how to deal with a variety of biohazards. 	LAFS.910.RI.1.1			
38.04	Use appropriate safety procedures and guidelines and demonstrate knowledge of emergency procedures. 	LAFS.910.RI.1.1			
38.05	Maintain equipment and material logs for all apparatus, materials, and technology. 				
38.06	Maintain Material Safety Data Sheet (MSDS) notebook and appropriately reference for each activity. 				
38.07	Follow approved protocols for all activities which may cause exposure to blood-borne pathogens.  				
38.08	Describe strategies used in a cleanroom to minimize the introduction of contaminating microorganisms or particulates.	LAFS.910.RI.1.1			
39.0	Recognize and follows quality control procedures and regulatory guidelines--The student will be able to:		SC.912.L.17.13,  14		
39.01	Describe the need for and function of regulatory agencies such as those in government, industry, and society. 	LAFS.910.RI.1.1			
39.02	Discuss quality control and assurance with respect to documentation. 	LAFS.910.RI.1.1			
39.03	Discuss quality control in relation to inspection results and specifications, procedures, testing methods, process control, regulatory specifications and documentation, and internal audits.	LAFS.910.RI.1.1			
39.04	Utilize quality control methods in relation to hazardous and non-hazardous materials. 				
40.0	Demonstrate the ability to communicate and use interpersonal skills effectively--The student will be able to:		SC.912.N.1.1		
40.01	Demonstrate ability to give and follow oral and written directions. 				
40.02	Recognize potential errors in protocol and address them with colleagues and the appropriate supervisor.	LAFS.910.SL.1.1			
40.03	Maintain thorough documentation of tasks and procedures.  	LAFS.910.W.2.4			
40.04	Work effectively in a research, manufacturing, quality control, or quality assurance team with a defined responsibility.				
40.05	Incorporate appropriate scientific terminology and abbreviations into all technical documents. 	LAFS.910.L.3.6			
40.06	Prepare, analyze, and discuss technical material. 	LAFS.910.RI.1.1			
41.0	Apply basic skills in scientific inquiry, calculations, and analysis--The student will be able to:		SC.912.N.1.1,  3,  4		
41.01	Develop scientific questions, hypotheses, and experimental plans. 	LAFS.910.RI.1.2 LAFS.910.W.2.4			
41.02	Properly and safely operate scientific equipment including mixers, analytical balances, stirrers, shakers, conductivity meters, and a hemocytometer. 				
41.03	Calculate ratios used for making chemical dilutions or plate counting. 				
41.04	Compose a thorough concluding statement outlining the results of an experiment with evidence, explanations, error analysis, and practical applications.  	LAFS.910.W.1.2			
41.05	Evaluate scientific reports with well-supported, clearly presented opinions. 	LAFS.910.W.1.1 LAFS.910.RI.1.1			
41.06	Consistently analyze and properly uses a variety of valid literature resources. 	LAFS.910.W.3.7			
41.07	Set-up and maintain a legal scientific notebook that includes an account of all laboratory procedures, data, conclusions, and appropriate signatures. 	LAFS.910.W.2.4			
41.08	Measure time, temperature, distance, capacity, mass/weight, flow rates and growth rates. 	MAFS.912.N-Q.1.3			
41.09	Use 24 hour time in all documents. 				
41.10	Create graphs, charts and tables used to record, analyze, and convey scientific data.	MAFS.912.S-ID.1.1,  2,  3 			
41.11	Critically analyze quantitative and qualitative data.  	MAFS.912.S-ID.1.1,  2,  3 			
41.12	Organize and communicate clear, concise written and oral reports of scientific findings. 	LAFS.910.W.1.2			
42.0	Demonstrate knowledge of organism structure and function--The student will be able to:		SC.912.L.15.15;  16.19; 18.1,  4 
SC.912.N.1.1		
42.01	Discuss the makeup of chromosomes. 	LAFS.910.RI.1.1			
42.02	Discuss the process of nucleic acid transfer. 	LAFS.910.RI.1.1			
42.03	Describe the relationship of cellular science and biotechnology. 	LAFS.910.RI.1.1			
42.04	Describe how enzymes regulate all aspects of protein synthesis. 	LAFS.910.RI.1.1			
42.05	Explain how the structure of nucleic acid affects its isolation from cells and solutions. 	LAFS.910.RI.1.1			
42.06	Describe how cells are engineered to express recombinant proteins.  	LAFS.910.RI.1.1			
42.07	Identify groups of proteins based on their functions, citing specific examples of proteins in each group. 	LAFS.910.RI.1.1			
42.08	Use the Internet to find information about the structure and function of specific proteins.	LAFS.910.W.3.7			
43.0	Utilize materials processing and standard laboratory operating procedures for biotechnology--The student will be able to:		SC.912.L.14.3,  6 SC.912.N.1.1		
43.01	Maintain a professional laboratory space following standard operating procedures. 				
43.02	Perform a variety of biological tests and chemical assays, collect data, perform calculations and statistical analysis.	MAFS.912.S-ID.1.1,  2,  3 			
43.03	Discuss classification, composition and preparation of culture media. 				
43.04	Discuss collection and handling of specimens for fungal, bacterial, mammalian cells and viral specimens, and parasites. 	LAFS.910.RI.1.1			
43.05	Prepare and examine specimens, and identify ova and parasites as indicated. 				
44.0	Apply biotechnical materials analysis skills--The student will be able to:		SC.912.L.14.3;  15.15; 16.3,  5,  9,  11,  12,  14;
17.13 
SC.912.N.1.1 SC.912.P.8.11		
44.01	Isolate DNA from cells and analyze its purity and concentration. 				
44.02	Outline the steps in production, product testing, and delivery of a product made through recombinant DNA technology. 	LAFS.910.RI.1.1			
44.03	Explain the principles involved in polyacrylamide and agarose gel electrophoresis.				
44.04	Prepare, load, run, visualize, and analyze protein samples on a polyacrylamide or agarose gel. 				
44.05	Prepare protein solutions and dilutions at specific concentrations and pH. 	MAFS.912.A-CED. 1.4			
44.06	Use protein indicator solutions to identify the presence and concentration of protein in solution. 				
44.07	Describe the meaning in differences in DNA and peptide bands seen on polyacrylamide or agarose gels. 	LAFS.910.W.1.2			
44.08	Prepare and maintain plate and broth cultures of bacteria.  Explain how polyacrilymide gel electrophoresis (PAGE) is used with column chromatography to monitor protein product. 	LAFS.910.RI.1.1 LAFS.910.SL.1.1			
44.09	Describe the steps in harvesting protein product from fermentation cell culture. 	LAFS.910.RI.1.1			
44.10	Outline the steps of using a visible light spectrophotometer.	LAFS.910.RI.1.1			
44.11	Prepare a serial dilution of protein and measure absorbance at a given wavelength.				
44.12	Use a standard curve to determine the concentration of an unknown protein solution.  	MAFS.912.S-ID.1.2, 3 			
44.13	Explain the protocol and application for isolating plasmids. 	LAFS.910.SL.2.4			
44.14	Explain the process and application of inserting genes that code for antibiotic resistance into a plasmid. 	LAFS.910.RI.1.1			
44.15	Demonstrate the ability to culture, propagate, and harvest bacteria. 				
44.16	Understand the bacterial growth stages in culture.	LAFS.910.RI.1.1			
44.17	Understand components of growth media such as energy source, and antibiotics, and incubation parameters: time, temp, atmospheric concentration.	LAFS.910.RI.1.1			
44.18	Explain the process of utilizing restriction enzymes and DNA ligase to insert a new gene into a plasmid. 	LAFS.910.RI.1.1			
44.19	Explain the process of replicating plasmids.	LAFS.910.RI.1.1			
44.20	Practice environmental monitoring using agar plates. 				
45.0	Demonstrates knowledge of basic chemistry as applied to biotechnology procedures--The student will be able to:		SC.912.P.8.4,  5,  7,  9, 11		
45.01	Use the periodic table to predict valence electron configuration, and physical and chemical characteristics of elements. 				
45.02	Use the periodic table to calculate molarity. 				
45.03	Balance equations to show that there is a conservation of matter.  Explain hydrogen and polar bonding. 	MAFS.912.A-CED. 1.1			
45.04	Discuss and use techniques that identify and separate components of a homogenous mixture. 	LAFS.910.SL.1.1			
45.05	Explain and use the function of pH in the preservation, purification, and functioning of proteins. 	LAFS.910.W.1.2			
45.06	Use pH paper or pH meter to measure and adjust pH. 				
45.07	Calculate how to prepare solutions based on % mass/volume.				
45.08	Calculate how to prepare solutions based on molar concentrations. 				
45.09	Use stoichiometry and molarity to prepare solutions of any volume and concentration. 	MAFS.912.A-CED. 1.4			
45.10	Prepare dilutions of concentrated solutions. 	MAFS.912.A-CED. 1.4			
46.0	Utilizes basic knowledge of microbiology and blood-borne diseases, including AIDS--The student will be able to:		SC.912.L.14.3,  4,  6,  16, 52;  15.5,  6;  16.9,  10; 17.1,  2,  4,  5		
46.01	Discuss bacterial metabolism, reproduction, cell structures and their functions. 	LAFS.910.RI.1.1			
46.02	Perform microbiology techniques in controlled environments. 				
46.03	Demonstrate techniques of microscope use related to oil immersion and slide preparation. 				
46.04	Discuss uses for different microscopy methods, including light, fluorescent, phase-contrast, and electron.	LAFS.910.RI.1.1			
46.05	Demonstrate the preparation and interpretation of Gram stains. 				
46.06	Perform various preparation and staining techniques. 				
46.07	Perform disinfection and aseptic techniques. 				
46.08	Perform sterilization and isolation techniques. 				
46.09	Prepare artificial culture media. 				
46.10	Perform techniques of inoculation and transfer of cultures. 				
46.11	Use various methods to monitor the growth of cell cultures. 				
46.12	Discuss the immune system and the normal immune response.	LAFS.910.RI.1.1			
46.13	Perform antigen and antibody testing. 				
46.14	Discuss methods that utilize the antigen/antibody complex as tools for research, diagnosis, and testing.	LAFS.910.RI.1.1			
46.15	Discuss the ABO, Rh and other blood group systems. 	LAFS.910.RI.1.1			
46.16	Distinguish between fact and fallacy about the transmission and treatment of diseases caused by blood borne pathogens including Hepatitis B. 				
46.17	Identify community resources and services available to the individuals with diseases caused by blood borne pathogens. 				
46.18	Demonstrate knowledge of the legal aspects of AIDS, including testing. 				
46.19	Describe how blood-borne pathogens are avoided in manufacturing. 				
47.0	Demonstrate knowledge of legal and ethical responsibilities--The student will be able to:		SC.912.L.16.10 SC.912.L.17.18		
47.01	Recognize ethical issues of the biotechnology workplace such as employee privacy, employee safety, animal testing, etc. 				
47.02	List the local, regional, state and federal agencies who oversee safety, ethics, and manufacturing. 				
47.03	Provide examples of the appropriate professional traits of a worker in biotechnology. 				
47.04	Outline the proper protocol for reporting unsafe or unethical behavior. 				
48.0	Demonstrate literacy and computer skills applicable to the biotechnology industry--The student will be able to: 				
48.01	Use the Internet to gather and share scientific and regulatory information. 	LAFS.910.W.2.6			
48.02	Use a variety of methods including literature searches in libraries, in computer databases, and on-line for gathering background information, making observations, and collecting and organizing data. 	LAFS.910.W.3.7			
48.03	Use a computer spreadsheet, word processing, and presentation programs to collect, analyze and report information or data. 	MAFS.912.S-ID.1.1,  2,  3,  4 			
49.0	Demonstrate employability skills--The student will be able to:				
49.01	Conduct a job search. 				
49.02	Develop a portfolio that demonstrates proficiency in specific biotechnology workplace tasks including writing samples and performance-based lab and computer skills.	LAFS.910.W.2.4			
49.03	Describe the opportunities for careers in biotechnology in health, industry, medicine, genetics, agriculture, etc. 				
49.04	Identify or demonstrate appropriate responses to criticism from employer, supervisor, or other persons. 				
49.05	Analyze the impact that work and health habits play in the biotechnology industry. 				
49.06	Recognize appropriate professional behavior. 				
49.07	Explain the roles of different departments and the employees within each department at an industry site. 				
49.08	Describe the departmental functions in a typical biotechnology company. 				

2014 – 2015
Florida Department of Education
Student Performance Standards

Course Title:		Biotechnology 3
Course Number:		8736030
Course Credit:		1

Biotechnology 3 is a course designed to provide students with intense laboratory and research skills in preparation for continued education and/or entry into the biotechnology industry.  Students will build upon the skills attained in Biotechnology 1 and Biotechnology 2 as they design and conduct experiments relevant to current biotechnology.  A close association with local industry and postsecondary institutions will provide additional opportunities for development and application of biotechnology practices.

Florida Standards	Correlation to CTE Program Standard #	
50.0	Methods and strategies for using Florida Standards for grades 11-12 reading in Technical Subjects for student success in Industrial Biotechnology.		
50.01	Key Ideas and Details		
50.01.1	Cite specific textual evidence to support analysis of science and technical texts, attending to important distinctions the author makes and to any gaps or inconsistencies in the account.         
LAFS.1112.RST.1.1		
50.01.2	Determine the central ideas or conclusions of a text; trace the text’s explanation or depiction of a complex process, phenomenon, or concept; provide an accurate summary of the text.
LAFS.1112.RST.1.2		
50.01.3	Follow precisely a complex multistep procedure when carrying out experiments, taking measurements, or performing technical tasks, attending to special cases or exceptions defined in the text.
LAFS.1112.RST.1.3		
50.02	Craft and Structure		
50.02.1	Determine the meaning of symbols key terms, and other domain-specific words and phrases as they are used in a specific scientific or technical context relevant to grades 11–12 texts and topics.
LAFS.1112.RST.2.4		
50.02.2	Analyze how the text structures information or ideas into categories or hierarchies, demonstrating understanding of the information or ideas.
LAFS.1112.RST.2.5		
50.02.3	Analyze the author’s purpose in providing an explanation, describing a procedure, or discussing an experiment in a text, identifying important issues that remain unresolved.                                 
LAFS.1112.RST.2.6		
50.03	Integration of Knowledge and Ideas		
50.03.1	Integrate and evaluate multiple sources of information presented in diverse formats and media (e.g. quantitative data, video, multimedia) in order to address a question or solve a problem.
LAFS.1112.RST.3.7		
50.03.2	Evaluate the hypotheses, data, analysis, and conclusions in a science or technical text, verifying the data when possible and corroborating or challenging conclusions with other sources of information.
LAFS.1112.RST.3.8		
50.03.3	Synthesize information from a range of sources (e.g., texts, experiments, simulations) into a coherent understanding of a process, phenomenon, or concept, resolving conflicting information when possible.
LAFS.1112.RST.3.9		
50.04	Range of Reading and Level of Text Complexity		
50.04.1	By the end of grade 11, read and comprehend literature [informational texts, history/social studies texts, science/technical texts] in the grades 11–CCR text complexity band proficiently, with scaffolding as needed at the high end of the range.
50.04.2	By the end of grade 12, read and comprehend literature [informational texts, history/social studies texts, science/technical texts] at the high end of the grades 11–CCR text complexity band independently and proficiently.
LAFS.1112.RST.4.10		
51.0	Methods and strategies for using Florida Standards for grades 11-12 writing in Technical Subjects for student success in Industrial Biotechnology.		
51.01	Text Types and Purposes		
51.01.1	Write arguments focused on discipline-specific content.
LAFS.1112.WHST.1.1		
51.01.2	Write informative/explanatory texts, including the narration of historical events, scientific procedures/experiments, or technical processes.
LAFS.1112.WHST.1.2		
51.02	Production and Distribution of Writing		
51.02.1	Produce clear and coherent writing in which the development, organization, and style are appropriate to task, purpose, and audience.
LAFS.1112.WHST.2.4		
51.02.2	Develop and strengthen writing as needed by planning, revising, editing, rewriting, or trying a new approach, focusing on addressing what is most significant for a specific purpose and audience.
LAFS.1112.WHST.2.5		
51.02.3	Use technology, including the Internet, to produce, publish, and update individual or shared writing products in response to ongoing feedback, including new arguments or information.
LAFS.1112.WHST.2.6		
51.03	Research to Build and Present Knowledge		
51.03.1	Conduct short as well as more sustained research projects to answer a question (including a self-generated question) or solve a problem; narrow or broaden the inquiry when appropriate; synthesize multiple sources on the subject, demonstrating understanding of the subject under investigation.
LAFS.1112.WHST.3.7		
51.03.2	Gather relevant information from multiple authoritative print and digital sources, using advanced searches effectively; assess the strengths and limitations of each source in terms of the specific task, purpose, and audience; integrate information into the text selectively to maintain the flow of ideas, avoiding plagiarism and overreliance on any one source and following a standard format for citation.
LAFS.1112.WHST.3.8		
51.03.3	Draw evidence from informational texts to support analysis, reflection, and research.
LAFS.1112.WHST.3.9		
51.04	Range of Writing		
51.04.1	Write routinely over extended time frames (time for reflection and revision) and shorter time frames (a single sitting or a day or two) for a range of discipline-specific tasks, purposes, and audiences.
LAFS.1112.WHST.4.10		
52.0	Methods and strategies for using Florida Standards for grades 11-12 Mathematical Practices in Technical Subjects for student success in Industrial Biotechnology.
		
52.01	Make sense of problems and persevere in solving them.
MAFS.K12.MP.1.1		
52.02	Reason abstractly and quantitatively.
MAFS.K12.MP.2.1		
52.03	Construct viable arguments and critique the reasoning of others.
MAFS.K12.MP.3.1		
52.04	Model with mathematics.
MAFS.K12.MP.4.1		
52.05	Use appropriate tools strategically.
MAFS.K12.MP.5.1		
52.06	Attend to precision.
MAFS.K12.MP.6.1		
52.07	Look for and make use of structure.
MAFS.K12.MP.7.1		
52.08	Look for and express regularity in repeated reasoning.
MAFS.K12.MP.8.1		

Abbreviations: 
FS-M/LA = Florida Standards for Math/Language Arts
NGSSS-Sci = Next Generation Sunshine State Standards for Science

Note:  This course is pending alignment in the following categories:  National Standards

CTE Standards and Benchmarks	FS-M/LA	NGSSS-Sci	National Standards	
CTE Performance Standards:				
53.0	History, career fields, and benefits of biotechnology--The student will be able to:		SC.912.L.17.15 SC.912.N.1.1,  4,  5;  2.2, 5		
53.01	Identify a recent advancement in a biotechnological tool or method, and compare it to its predecessor.	LAFS.1112.W.3.7			
53.02	Select and evaluate the potential of a product based on industry criteria. 				
53.03	Explain how biotechnology practices, procedures, and philosophies have evolved to current high technology and integrated disciplines.				
53.04	Illustrate examples of how biotechnology has led to benefits and risks to society and how biotechnical advances affect human lives on a personal level. 	LAFS.1112.W.1.2 LAFS.1112.RI.1.1			
53.05	Graph a history timeline with medical advances due to technology advances. 				
53.06	Research and analyze career opportunities available in biotechnology and select the career pathway best suited to your interests, abilities, and objectives. 	LAFS.1112.W.3.7			
53.07	Discuss medical, agricultural, forensic, and environmental applications of biotechnology. 	LAFS.1112.RI.1.1			
54.0	Safety procedures--The student will be able to:		SC.912.L.14.6;  16.10; 17.16,  20 
SC.912.N.1.1,  2;  4.2		
54.01	Identify safety symbols and signs. 				
54.02	Use appropriate safety procedures and guidelines. 				
54.03	Demonstrate an understanding of the emergency procedures in case of fire, burn, chemical spill or other hazardous situations.				
54.04	Recognize laboratory safety hazards and avoid them. 				
54.05	Locate and be able to use emergency equipment, including first aid. 				
54.06	Identify potential biohazards and relate how to deal with a variety of biohazards. 				
54.07	Use laboratory apparatus, materials, and technology in an appropriate and safe manner. 				
54.08	Locate a Material Safety Data Sheet (MSDS) and use the information to operate in a safe manner.  				
54.09	Follow universal precautions for blood-borne pathogens. 				
55.0	Quality control procedures and regulatory guidelines--The student will be able to:		SC.912.L.17.13,  20 SC.912.N.1.1,  2;  2.2		
55.01	Evaluate the need for and function of regulatory agencies such as those in government, industry, and society.				
55.02	Understand that all products intended to be used for the diagnosis, cure, mitigation, treatment, or prevention of disease, must go through a regulatory approval process that is based on documented research and testing to ensure the product is safe and efficacious (works).				
55.03	Describe the purpose of current Good Manufacturing Practices, and how they are supported by guidances from the International Organization for Standardization.				
55.04	Analyze experimental data and/or manufacturing processing documentation from the perspective of quality assurance. 				
55.05	Discuss quality control in relation to inspection results and specifications, procedures, testing methods, process control, regulatory specifications and documentation, and internal audits. 				
55.06	Monitor, use, store and dispose of hazardous materials properly. 				
55.07	Check and maintain equipment and logs.				
55.08	Clean, organize, and sterilize materials.				
55.09	Manage material and supply inventories. 				
55.10	Define/chart the process of receiving an unqualified (or qualified) raw material and follow it through the manufacturing process into the finished product.				
56.0	Communicate and use interpersonal skills effectively--The student will be able to:		SC.912.L.17.13 SC.912.N.1.1,  3,  7;  2.1, 2,  4,  5;  3.1		
56.01	Follow all oral and written instructions.				
56.02	Demonstrate ability to give oral and written directions. 				
56.03	Demonstrate good listening, writing, and verbal communication skills and procedures. 	LAFS.1112.W.2.4 LAFS.1112.SL.1.1			
56.04	Recognize potential errors in protocol and address them with colleagues and the appropriate supervisor. 				
56.05	Maintain thorough documentation of tasks and procedures.				
56.06	Work effectively in a research, manufacturing, quality control, or quality assurance team with a defined responsibility. 				
56.07	Develop basic observational skills and related documentation strategies in written and oral form. 	LAFS.1112.W.2.4 LAFS.1112.SL.1.1			
56.08	Identify characteristics of successful and unsuccessful communication. 				
56.09	Appropriately use and respond to verbal and non-verbal cues.				
56.10	Use correct spelling, grammar, and format in all written communication. 	LAFS.1112.L.1.2			
56.11	Use appropriate scientific terminology and abbreviations.	LAFS.1112.L.3.6			
56.12	Recognize the importance of courtesy and respect and maintain good interpersonal relationships.  				
56.13	Interpret technical material and prepare a journal article related to the development of a process or product on which the student has worked. 	LAFS.1112.W.1.2			
57.0	Basic skills in scientific inquiry, calculations, and analysis--The student will be able to:		SC.912.L.14.4 SC.912.N.1.1,  2,  3,  4, 5,  6,  7;  2.1,  2;  3.1,  4, 5;  4.1,  2		
57.01	Demonstrate knowledge of the scientific method in general and biotechnology research in manufacturing and quality control. 				
57.02	Understand the applicability of Koch’s postulates.				
57.03	Consistently analyze and properly uses valid literature resources.	LAFS.1112.RI.1.1			
57.04	Set-up and maintain a legal scientific notebook that includes an account of all laboratory procedures, data, conclusions, and appropriate signatures.  	LAFS.1112.W.2.4			
57.05	Properly and safely operate scientific equipment including graduates, hoods, microscopes, pipets, micropipets, electronic balance, pH meters, incubators, centrifuges, water baths, power supplies and electrophoresis chambers.  				
57.06	Properly and safely operate scientific equipment including mixers, analytical balances, stirrers, shakers, conductivity meters, and a hemocytometer. 				
57.07	Describe how to properly and safely use scientific equipment including spectrophotometer, autoclave, thermocycler, plate reader/fluorometer, and sterile hood/ biosafety cabinet.  				
57.08	Understand why sometimes equipment is dedicated to one specific room, assay, or process.				
57.09	Describe how to properly and safely use scientific equipment including polarimeter, chart recorder, particle counter, and hybridization oven.				
57.10	Make and use measurements in both traditional and metric units. 	MAFS.912.N-Q.1.3			
57.11	Calculate ratios used for making chemical dilutions or plate counting. 				
57.12	Measure time, temperature, distance, capacity, mass/weight, flow rates and growth rates. 	MAFS.912.N-Q.1.3			
57.13	Describe the relationship between 12 and 24 hour time and be able to convert between the two.				
57.14	Make estimates and approximations in order to test the reasonableness of the result. 	MAFS.912.N-Q.1.3			
57.15	Evaluate the validity of results obtained during experimentation and product development. 	LAFS.1112.SL.1.1			
57.16	Interpret and create graphs, charts and tables used to collect and analyze data.  	MAFS.912.S-ID.1.1,  2,  3			
57.17	Interpret and critically analyze quantitative and qualitative data.  	MAFS.912.S-ID.1.1,  2,  3			
57.18	Demonstrate ability to evaluate and draw conclusions. 				
57.19	Organize and communicate clear, concise written and oral reports of scientific findings. 	LAFS.1112.W.1.2			
57.20	Evaluate scientific reports with well-supported, clearly presented opinions. Monitor scientific equipment by conducting and documenting preventative maintenance and calibration. 	LAFS.1112.RI.1.1 LAFS.1112.SL.1.1			
58.0	Organism structure and function--The student will be able to:		SC.912.L.14.2,  3;  16.3, 4,  5,  6,  7,  9,  10,  11, 12;  18.1,  4,  11		
58.01	Discuss the makeup of chromosomes. 	LAFS.1112.RI.1.1			
58.02	Discuss the process of nucleic acid transfer.  	LAFS.1112.RI.1.1			
58.03	Describe the relationship of cellular science and biotechnology. 	LAFS.1112.RI.1.1			
58.04	Explain how the structure of nucleic acid affects its isolation from cells and solutions. 	LAFS.1112.RI.1.1			
58.05	Describe how cells are engineered to express recombinant proteins.  	LAFS.1112.RI.1.1			
59.0	Materials processing and standard laboratory operating procedures for biotechnology--The student will be able to:		SC.912.L.14.9;  15.4,  5, 6,  7;  18.1,  2,  3,  4 
SC.912.N.1.1		
59.01	Maintain a clean and organized work area.				
59.02	Follow written protocols and oral directions to perform a variety of laboratory and technical tasks. 				
59.03	Determine appropriate equipment and units of measurement for a given task. 	MAFS.912.N-Q.1.3			
59.04	Discuss the various sections of a Standard Operating Procedure (SOP), with respect to safety, equipment, reagents, procedural steps, result analysis, reporting, and troubleshooting.				
59.05	Perform a variety of biological tests and chemical assays, collect data, perform calculations and statistical analysis.	MAFS.912.S-ID.1.1, 2, 3			
59.06	Discuss and perform disinfection and sterilization techniques.				
59.07	Discuss classification, composition and preparation of culture media. 				
59.08	Discuss collection and handling of specimens for fungal, bacterial, mammalian cells and viral specimens. 				
59.09	Prepare and examine specimens, and identify ova and parasites as indicated. 				
60.0	Biotechnical materials analysis skills--The student will be able to:		SC.912.L.14.2,  6,  9; 15.15;  16.2,  3,  5,  7,  10,  12;  17.13;  18.1,  4, 8,  11,  14 
SC.912.N.1.1 SC.912.P.10.1,  10,  18, 19,  22;  12.12		
60.01	Outline the steps in cell culture, sterile technique, and media preparation. 	LAFS.1112.RI.1.1			
60.02	Describe the characteristics of proteins that allow for their purification after cloning transformed cells.	LAFS.1112.RI.1.1			
60.03	Explain how polyacrilymide gel electrophoresis (PAGE) is used with column chromatography to monitor protein product. 	LAFS.1112.RI.1.1			
60.04	Describe the steps in harvesting protein product from fermentation cell culture. 	LAFS.1112.RI.1.1			
60.05	Summarize the steps in manufacturing and product testing and FDA approval for new drugs produced through genetic engineering. 	LAFS.1112.RI.1.2			
60.06	Outline the steps of using a visible light spectrophotometer.				
60.07	Prepare a serial dilution of protein and measure absorbance at a given wavelength. 				
60.08	Use a standard curve to determine the concentration of an unknown protein solution. 	MAFS.912.S-ID.1.2,  3			
60.09	Do a linear regression to calculate protein concentration using a computer spreadsheet. 				
60.10	Discuss techniques of chemistry related to standardization of procedure and use of standards, blanks and controls. 				
60.11	Explain the protocol and application for isolating plasmids.	LAFS.1112.RI.1.1			
60.12	Explain the process and application of inserting genes that code for antibiotic resistance into a plasmid. 	LAFS.1112.RI.1.2			
60.13	Demonstrate the ability to culture, propagate, and harvest bacteria. 				
60.14	Explain the process of utilizing restriction enzymes and DNA ligase to insert a new gene into a plasmid. 	LAFS.1112.RI.1.2			
60.15	Explain the process of replicating plasmids. 	LAFS.1112.RI.1.2			
60.16	Practice environmental monitoring using agar plates. 				
60.17	Discuss sources of environmental contamination and methods of detection in controlled environments. 				
60.18	Demonstrate knowledge of and perform enzyme assays.				
61.0	Basic chemistry as applied to biotechnology procedures--The student will be able to:		SC.912.N.1.1 SC.912.P.8.2,  5 , 6,  7,  8,  9,  11		
61.01	Use the periodic table to predict valence electron configuration, and physical and chemical characteristics of elements. 				
61.02	Use the periodic table to calculate molarity.  				
61.03	Balance equations to show that there is a conservation of matter. 	MAFS.912.A-CED. 1.1			
61.04	Explain hydrogen and polar bonding. 	LAFS.1112.L.3.6			
61.05	Discuss and use techniques that identify and separate components of a homogenous mixture. 	LAFS.1112.SL.1.1			
61.06	Explain and use the function of pH in the preservation, purification, and functioning of proteins. 				
61.07	Use pH paper or pH meter to measure and adjust pHSC.				
61.08	Calculate how to prepare solutions based on % mass/volume. 				
61.09	Calculate how to prepare solutions based on molar concentrations. 				
61.10	Use stoichiometry and molarity to prepare solutions of any volume and concentration.	MAFS.912.A-CED. 1.4			
61.11	Prepare dilutions of concentrated solutions. 	MAFS.912.A-CED. 1.4			
62.0	Microbiology and blood-borne diseases, including AIDS--The student will be able to:		SC.912.L.14.1, 2, 3, 6, 52; 15.5;  16.10,  11,  12, 13,  14,  15,  16,  17,  18;
17.8 
SC.912.N.1.1,  4,  6,  7		
62.01	Discuss microbial taxonomy and classification. 	LAFS.1112.RI.1.1			
62.02	Perform microbiology techniques in controlled environments.				
62.03	Perform disinfection techniques. 				
62.04	Practice aseptic techniques as required. 	MAFS.912.S-ID.1.1,  2,  3,  4			
62.05	Perform sterilization techniques. 				
62.06	Discuss isolation techniques. 	LAFS.1112.RI.1.1			
62.07	Prepare artificial culture media.				
62.08	Discuss techniques of inoculation and transfer of cultures	LAFS.1112.RI.1.1			
62.09	Use various methods to monitor the growth of cell cultures. 				
62.10	Describe conditions that promote cell growth under aseptic conditions in the laboratory and workplace. 	LAFS.1112.RI.1.1			
62.11	Discuss methods for the isolation, purification, and quantification of DNA and plasmid DNA.	LAFS.1112.RI.1.1			
62.12	Perform antigen and antibody testing. 				
62.13	Identify community resources and services available to the individuals with diseases caused by blood borne pathogens.				
62.14	Demonstrate knowledge of the legal aspects of AIDS, including testing. 				
62.15	Describe how blood-borne pathogens are avoided in manufacturing. 				
63.0	Legal and ethical responsibilities--The student will be able to:		SC.912.L.16.10 SC.912.N.1.2,  5,  6,  7; 2.3,  5		
63.01	Investigate an ethical, social, or legal issues facing biotechnology today and suggest an approach to solving it. 	LAFS.1112.W.3.7			
63.02	Provide examples of the appropriate professional traits of a worker in biotechnology. 				
63.03	Outline the proper protocol for reporting unsafe or unethical behavior. 				
63.04	Describe a Code of Ethics consistent with the biotechnology industry 				
63.05	Discuss the importance of maintaining confidentiality of information, including computer information. 				
63.06	Recognize and report illegal and unethical practices of health care workers. 				
64.0	Literacy and computer skills applicable to the biotechnology industry--The student will be able to:		SC.912.N.1.1,  4;  2.3,  4; 3.5		
64.01	Use the Internet to gather and share scientific and regulatory information. 	LAFS.1112.W.3.7			
64.02	Use a computer spreadsheet, word processing, and presentation programs to collect, analyze and report information or data.				
64.03	Use a variety of methods including literature searches in libraries, in computer databases, and on-line for gathering background information, making observations, and collecting and organizing data.				
65.0	Employability skills--The student will be able to:		SC.912.L.16.10 SC.912.N.1.1,  4,  6,  7		
65.01	Conduct a job search. 				
65.02	Use several resources including the Internet to gather information about job opportunities in the biotechnology field. 	LAFS.1112.W.3.7			
65.03	Create an appropriate resume for use in applying for job opportunities in a biotechnology company. 	LAFS.1112.W.2.4			
65.04	Use ‘keywords’ in a resume to match job description and rank higher when analyzed by resume scanning software.				
65.05	Complete a job application form correctly.				
65.06	Identify documents that may be required when applying for a job.				
65.07	Develop a portfolio that demonstrates proficiency in specific biotechnology workplace tasks including writing samples and performance-based lab and computer skills. 	LAFS.1112.W.1.2			
65.08	Identify or demonstrate appropriate responses to criticism from employer, supervisor, or other persons. 				
65.09	Evaluate the overall condition of personal work habits and health habits and the effect, (if any) these habits might have in the biotechnology workplace. 				
65.10	Recognize appropriate professional behavior.				
65.11	Explain the roles of different departments and the employees within each department at an industry site.				
65.12	Describe the departmental functions in a typical biotechnology company. 				
65.13	Demonstrate knowledge of how to make job changes appropriately.				

Additional Information

Laboratory Activities

This program is designed to provide students with intense laboratory and research skills in preparation for continued education and/or entry into the biotechnology industry.  Students will build upon the skills attained as they design and conduct experiments relevant to current biotechnology.  A close association with local industry and postsecondary institutions will provide additional opportunities for development and application of biotechnology practices.

Laboratory investigations that include scientific inquiry, research, measurement, problem solving, emerging technologies, tools and equipment, as well as, experimental, quality, and safety procedures are an integral part of this career and technical program/course. Laboratory investigations benefit all students by developing an understanding of the complexity and ambiguity of empirical work, as well as the skills required to manage, operate, calibrate and troubleshoot equipment/tools used to make observations. Students understand measurement error; and have the skills to aggregate, interpret, and present the resulting data. Equipment and supplies should be provided to enhance hands-on experiences for students.

Career and Technical Student Organization (CTSO)

SkillsUSA is the appropriate career and technical student organization for providing leadership training and reinforcing specific career and technical skills.  Career and Technical Student Organizations provide activities for students as an integral part of the instruction offered.  The activities of such organizations are defined as part of the curriculum in accordance with Rule 6A-6.065, F.A.C.

Cooperative Training – OJT

On-the-job training is appropriate but not required for this program.  Whenever offered, the rules, guidelines, and requirements specified in the OJT framework apply. 

Accommodations

Federal and state legislation requires the provision of accommodations for students with disabilities as identified on the secondary student’s Individual Educational Plan (IEP) or 504 plan or postsecondary student’s accommodations’ plan to meet individual needs and ensure equal access.  Postsecondary students with disabilities must self-identify, present documentation, request accommodations if needed, and develop a plan with their counselor and/or instructors.  Accommodations received in postsecondary education may differ from those received in secondary education.  Accommodations change the way the student is instructed.  Students with disabilities may need accommodations in such areas as instructional methods and materials, assignments and assessments, time demands and schedules, learning environment, assistive technology and special communication systems.  Documentation of the accommodations requested and provided should be maintained in a confidential file.

In addition to accommodations, some secondary students with disabilities (students with an IEP served in Exceptional Student Education (ESE)) will need modifications to meet their needs.  Modifications change the outcomes or what the student is expected to learn, e.g., modifying the curriculum of a secondary career and technical education course.  Note: postsecondary curriculum and regulated secondary programs cannot be modified.

Some secondary students with disabilities (ESE) may need additional time (i.e., longer than the regular school year), to master the student performance standards associated with a regular Occupational Completion Point (OCP) or a Modified Occupational Completion Point (MOCP).  If needed, a student may enroll in the same career and technical course more than once.  Documentation should be included in the IEP that clearly indicates that it is anticipated that the student may need an additional year to complete an OCP/MOCP.  The student should work on different competencies and new applications of competencies each year toward completion of the OCP/MOCP.  After achieving the competencies identified for the year, the student earns credit for the course.  It is important to ensure that credits earned by students are reported accurately.  The district’s information system must be designed to accept multiple credits for the same course number for eligible students with disabilities.

Articulation

For details on articulation agreements which correlate to programs and industry certifications refer to http://www.fldoe.org/workforce/dwdframe/artic_frame.asp.

Bright Futures/Gold Seal Scholarship

Course substitutions as defined in the Comprehensive Course Table for this program area may be used to qualify a student for Florida’s Gold Seal Vocational Scholarship, providing all other eligibility requirements are met.  Eligibility requirements are available online at https://www.osfaffelp.org/bfiehs/fnbpcm02_CCTMain.aspx. 

Fine Arts/Practical Arts Credit

Many courses in CTE programs meet the Fine Arts/Practical Arts credit for high school graduation (http://www.fldoe.org/articulation/CCD/files/pacourses1314.pdf).  A listing of approved CTE courses is published each year as a supplemental resource to the Course Code Directory (http://www.fldoe.org/articulation/CCD/default.asp).
 
Equivalent Mathematics and Equally Rigorous Science Courses

Equally rigorous science courses are based upon levels of cognitive complexity of content specific benchmarks, depth and breadth of content focus, and required laboratory components. 

Courses in this program satisfying equally rigorous science content are:

•	Biotechnology 1 (3027010)
•	Biotechnology 2 (3027020)