2014 – 2015
Florida Department of Education
Curriculum Framework 

Program Title:	Biotechnology
Career Cluster:	Manufacturing

AS	
CIP Number	1626120100	
Program Type	College Credit	
Standard Length	61 credit hours	
CTSO	SkillsUSA	
SOC Codes (all applicable) 	19-4021 – Biological Technicians	
Targeted Occupation List	http://www.labormarketinfo.com/wec/TargetOccupationList.htm	
Perkins Technical Skill Attainment Inventory	http://www.fldoe.org/workforce/perkins/perkins_resources.asp 	
Statewide Articulation	http://www.fldoe.org/workforce/dwdframe/artic_frame.asp 	

Purpose

This program offers a sequence of courses that provides coherent and rigorous content aligned with challenging academic standards and relevant technical knowledge and skills needed to prepare for further education and careers in the manufacturing career cluster; provides technical skill proficiency, and includes competency-based applied learning that contributes to the academic knowledge, higher-order reasoning and problem-solving skills, work attitudes, general employability skills, technical skills, and occupation-specific skills, and knowledge of all aspects of the manufacturing career cluster. 

The content includes but is not limited to communication skills, leadership skills, human relations and employability skills, safe and efficient work practices, and a combination of theory and laboratory activities to gain the necessary cognitive and manipulative skills to perform preventive and corrective maintenance, engineering support, and maintain product quality.

Additional Information relevant to this Career and Technical Education (CTE) program is provided at the end of this document.

Program Structure

This program is a planned sequence of instruction consisting of sixty-one credit hours.

Program Length

The AS degree requires the inclusion of a minimum of 15 credits of general education coursework according to SACS, and it must be transferable according to Rule 6A-14.030 (2), F.A.C.  The standard length of this program is sixty-one credit hours according to Rule 6A-14.030, F.A.C.

Standards

After successfully completing this program, the student will be able to perform the following:

01.0	Demonstrate communication skills.
02.0	Demonstrate safety skills.
03.0	Demonstrate basic laboratory skills.
04.0	Demonstrate regulatory compliance.
05.0	Demonstrate appropriate decision making and problem solving techniques.
06.0	Demonstrate specific laboratory skills.
07.0	Demonstrate quality assurance/control.
08.0	Maintain facility and equipment.
09.0	Demonstrate knowledge and proper care/use of test animals/plants.

2014 – 2015
Florida Department of Education
Student Performance Standards

Program Title:	Biotechnology
CIP Numbers:	1626120100
Program Length:	61 credit hours
SOC Code(s):	19-4021

The AS degree requires the inclusion of a minimum of 15 credits of general education coursework according to SACS, and it must be transferable according to Rule 6A-14.030 (2), F.A.C.  At the completion of this program, the student will be able to:	
01.0	Demonstrate communication skills--The student will be able to:	
01.01	Make professional oral and written presentations.	
01.02	Comprehend and use correct technical vocabulary.	
01.03	Follow/analyze experimental and lab protocols.	
01.04	Keep accurate lab records in notebooks. (hand written and electronically)	
01.05	Review and maintain notes on procedures. (hand written and electronically)	
01.06	Prepare identify and apply changes to control procedures.	
01.07	Write or update manuals, SOP’s protocols, reports and technical summaries.	
01.08	Perform computerized research and web searches, including, but not limited to Pub Med.	
01.09	Read technical literature, including, but not limited to original research articles.	
01.10	Identify basic reference resources in biotechnology, including, but not limited to original journal articles.	
01.11	Perform basic applications in word processing, spread sheets, databases, presentations and project management.	
01.12	Make professional oral and written presentations.	
02.0	Demonstrate safety skills--The student will be able to:	
02.01	Identify first aid supplies, eye wash station, emergency shower, co-worker contact, medical information, and emergency protection and evacuation plan.	
02.02	Follow correct safety procedures, guidelines and chemical hygiene plans.	
02.03	Maintain required environmental health & safety, and lab animal training.	
02.04	Maintain a safe, uncluttered and clean work area.	
02.05	Maintain and utilize safety equipment and personal protection equipment.	
02.06	Check expiration dates, lot numbers and labels for chemical and hazardous substances.	
02.07	Monitor usage and exposure to hazardous materials, and keep appropriate usage logs.	
02.08	Handle, store and dispose of waste and hazardous materials per SDS, other safety guidelines and Worker Protection Standards (WPS).	
02.09	Follow universal precautions for biological pathogens, both proper handling and disposal.	
02.10	Store and label chemicals and biologicals according to industry recognized storage guidelines.	
03.0	Demonstrate basic laboratory skills--The student will be able to:	
03.01	Obtain and read protocol, test procedure, standard operating procedure (SOP), equipment manuals, and proper forms.	
03.02	Prioritize and perform multiple tasks in a timely manner, based upon priorities communicated by supervisor.	
03.03	Clean, organize and sterilize materials and lab instruments, when required.	
03.04	Check and maintain equipment, logs and perform preventative maintenance tasks according to schedule.	
03.05	Order inventory of supplies; date/label reagents. Store promptly upon arrival.	
03.06	Practice aseptic technique.	
03.07	Use titration/pipetting techniques; measure volume/weights precisely.	
03.08	Perform basic calculations and statistical analysis using appropriate software.	
03.09	Calculate and prepare dilutions series.	
03.10	Prepare solutions and reagents for laboratory use.	
03.11	Monitor physical properties of reagents, buffers, media and solutions and determine optimum conditions for use.	
03.12	Obtain and review appropriate procedures and test forms, prepare for lab inspections and respond to the reports.	
03.13	Collect and set up samples for analysis.	
03.14	Set up general laboratory tests, including, setup equipment and perform/document tests and results.	
03.15	Operate laboratory equipment and instrumentation after familiarization with manuals and or training, which may include the following, but not limited to: Thermocycler, microscopes, fluorimeter, hoods, centrifuge, polarimeter, pH meter, chart recorder, stirrers, balance, conductivity meter, mixers, autoclave, power supply, shakers, dry heat ovens, incubator, Bunsen burner, scintillation counter, high pressure liquid chromatography, gas chromatography/mass spectrometry.	
04.0	Demonstrate regulatory compliance--The student will be able to:	
04.01	Follow guidelines from the appropriate regulatory, accreditation, and/or certification agencies, such as FDA, OSHA, USDA, NIH, NR, DOT, EPA, CDC, ISO/IEC and NRC.	
04.02	Accept and follow state, local and industry regulations.	
04.03	Perform manufacturing processes using current continuous quality improvement practices.	
05.0	Demonstrate appropriate decision making and problem solving techniques--The student will be able to:	
05.01	Identify decision to be made, compare alternatives, and discuss alternatives with supervisor.	
05.02	Apply decision making skills in the workplace.	
05.03	Make decisions based on accurate facts, data, and agreed-upon goals.	
05.04	Evaluate the decision made quantitatively and qualitatively.	
05.05	Apply problem solving techniques in the workplace.	
05.06	Diagnose problem, its urgency and causes, and documenting as appropriate.	
05.07	Compare and contrast advantages/disadvantages for solutions to a problem.	
05.08	Determine appropriate action; implement it and evaluate results.	
06.0	Demonstrate specific laboratory skills--The student will be able to:	
06.01	Identify and quantify microorganisms and cells using manual and automated systems.	
06.02	Isolate, maintain and store pure cell cultures using incubators.	
06.03	Prepare seed inoculum.	
06.04	Harvest cells and recover effluent products.	
06.05	Transform cells.	
06.06	Perform bioassays.	
06.07	Decontaminate and/or dispose of equipment, glassware, biologicals.	
06.08	Perform microbiology skills, which may include but are not limited to, plating techniques, isolating and characterizing cell lines, propagating cell lines, and cryogenic techniques.	
06.09	Apply cell biology and anatomical/histological, which may include but are not limited to, transfection techniques, monoclonal and polyclonal antibodies, and hemagglutination/hemadsorption techniques.	
06.10	Perform immunological techniques, which may include but are not limited to, enzyme-linked immunoabsorbent assays, probe and analyze DNA library, transformation techniques, polymerase chain reaction, translation assays, and construct recombinant vectors.	
06.11	Perform genetic engineering and molecular biology techniques, which may include but are not limited to, isolate and analyze nucleic acid isolation, non-isotope techniques, disrupt cells, protein gels, Western blotting, denature, renature proteins, precipitate soluble proteins, concentrate(filter and dialyze) proteins, quantitative proteins, and enzyme activity assays.	
06.12	Perform additional specific skills, which may include but are not limited to transcribing DNA, electrophoresis on RNA, DNA and proteins, and nucleic acid hybridization.	
06.13	Separate, isolate or characterize proteins, which may include but are not limited to, monitoring protein stability, quantitative analysis and distillation techniques, titration techniques, employing dyes and indicators, lypholization and organic chemistry techniques, and perform extractions.	
06.14	Perform chemical and physical assays including measuring turbidity, viscosity, and density.	
07.0	Demonstrate quality assurance/control--The student will be able to:	
07.01	Perform quality tests and document results.	
07.02	Verify test standards and maintain QA records.	
07.03	Archive samples and documents.	
07.04	Inspect and verify integrity of product, procedure, and specimen.	
07.05	Release final product and perform trend analysis.	
07.06	Investigate complaints and take corrective action.	
08.0	Maintain facility and equipment--The student will be able to:	
08.01	Monitor/record the environmental condition of the facility (growth chamber, laboratory, greenhouse, seed storage room, animal room or manufacturing site).	
08.02	Notify appropriate personnel if sampling indicates a problem.	
08.03	Clean work area according to SOPs.	
08.04	Label equipment.	
08.05	Check calibration and perform systems diagnostics	
08.06	Perform or schedule preventive maintenance.	
08.07	Maintain equipment logs.	
09.0	Demonstrate knowledge and proper care/use of test animals/plants--The student will be able to:	
09.01	Receive and transport animals.	
09.02	Maintain separate in-process, quarantine and release areas. 	
09.03	Feed, water and monitor animals, including separating males/females.	
09.04	Prepare animal food and prescription diets.	
09.05	Clean housing and sterilize cages.	
09.06	Monitor animal health and keep health records.	
09.07	Follow USDA/IACUC guidelines for animal use and care.	
09.08	Properly restrain and handle animals.	
09.09	Collect and process specimens; collect data and analyze document results.	
09.10	Maintain plants for optimal growth.	
09.11	Apply agrochemical safely.	
09.12	Maintain and monitor insect populations.	
09.13	Maintain plant growth media.	
09.14	Inoculate plant and/or soil with biological materials.	
09.15	Gather pollen and bundle pollinate.	
09.16	Apply plant pests safely.	
09.17	Collect data, perform bioassays, and document results of test plants.	

Additional Information

Laboratory Activities

Laboratory activities are an integral part of this program.  These activities include instruction in the use of safety procedures, tools, equipment, materials, and processes related to these occupations.  Equipment and supplies should be provided to enhance hands-on experiences for students. 

Career and Technical Student Organization (CTSO)

SkillsUSA is the appropriate career and technical student organization for providing leadership training and reinforcing specific career and technical skills.  Career and Technical Student Organizations provide activities for students as an integral part of the instruction offered.  The activities of such organizations are defined as part of the curriculum in accordance with Rule 6A-6.065, F.A.C.

Accommodations

Federal and state legislation requires the provision of accommodations for students with disabilities as identified on the secondary student’s Individual Educational Plan (IEP) or 504 plan or postsecondary student’s accommodations’ plan to meet individual needs and ensure equal access.  Postsecondary students with disabilities must self-identify, present documentation, request accommodations if needed, and develop a plan with their counselor and/or instructors.  Accommodations received in postsecondary education may differ from those received in secondary education.  Accommodations change the way the student is instructed.  Students with disabilities may need accommodations in such areas as instructional methods and materials, assignments and assessments, time demands and schedules, learning environment, assistive technology and special communication systems.  Documentation of the accommodations requested and provided should be maintained in a confidential file.

Articulation

To be transferable statewide between institutions, this program must have been reviewed, and a “transfer value” assigned the curriculum content by the appropriate Statewide Course Numbering System discipline committee.  This does not preclude institutions from developing specific articulation agreements with each other. 

For details on articulation agreements which correlate to programs and industry certifications refer to http://www.fldoe.org/workforce/dwdframe/artic_frame.asp.

Certificate Programs

A College Credit Certificate consists of a program of instruction of less than sixty (60) credits of college-level courses, which is part of an AS or AAS degree program and prepares students for entry into employment (Rule 6A-14.030, F.A.C.).  This AS degree program includes the following College Credit Certificates:
	
	Biotechnology Specialist (0626120101) – 19 credit hours 

Standards for the above certificate programs are contained in separate curriculum frameworks.